Fig. 3

AtNLP7^ΔGAF loses its ability to bind NRE and fails to activate the expression of its target genes. A, B ChIP–qPCR enrichment of NRE-containing promoter fragments (relative to input) from AtNIR1 (A) and AtNIA1 (B) in different AtNLP7 domain deletion mutants. Data are mean ± SD (n = 3). The letters indicate significant differences (P < 0.05). C Schematic representation of the effector and reporter constructs used in the transactivation assay. D Transient transactivation assays. Different AtNLP7 domain deletion variants were used to activate AtNIR1 promoter-LUC fusion construct as described in the Materials and Methods. Renilla luciferase gene (REN) used as an internal control. Data are mean ± SD (n = 3). The letters indicate significant differences (P < 0.05)