Skip to main content
Fig. 2 | BMC Plant Biology

Fig. 2

From: GAF domain is essential for nitrate-dependent AtNLP7 function

Fig. 2

Effects of mutations in different domains of AtNLP7 on nitrate-inducible gene expression and nitrate assimilation ability. A-F Seedlings of WT, nlp7-1 and the complementation lines were grown on N-free medium for 7 days and then treated with 10 mM KNO3 for 1 h. Transcript levels were normalized against AtUBQ5 expression. RT-qPCR data are mean ± SD (n = 3). P values are from the one-way ANOVA (The letters indicate significant differences. P < 0.05). NRT2.1, nitrate transporter 2.1; NIA1, nitrate reductase 1; NIR1, nitrite reductase 1; GS2, glutamine synthetase 2; LBD, lateral organ boundary domain. G 15NO3 uptake activity assay. 10-day-old seedlings were labeled with 5 mM 15NO3 for 1 h and the amount of 15NO3 taken into the plants was measured. Values are the mean ± SD of three replications each containing 30 plants per genotype (The letters indicate significant differences. P < 0.05). DW, dry weight. H, I Content of nitrate (H) and enzyme activity of nitrate reductase (I) in the plants grown under different nitrate conditions. 14-day-old seedlings grown on agar medium with 1 mM (LN) or 10 mM (HN) KNO3 were used for metabolite analyses as described in Material and Methods. Values are the mean ± SD of three replications each containing 15 plants per genotype (The letters significant differences. P < 0.05). FW, fresh weight

Back to article page