Table 4 Real Time PCR reaction mixture
Compound | Amount (μL) |
|---|---|
cDNA (1:10 diluted) | 6 |
Siber Green qPCR Mix | 4 |
Forward primer (10 μM) | 5 |
Reverse primer (10 μM) | 5 |
DEPC water | 10 |
Total volume | 30 |
Compound | Amount (μL) |
|---|---|
cDNA (1:10 diluted) | 6 |
Siber Green qPCR Mix | 4 |
Forward primer (10 μM) | 5 |
Reverse primer (10 μM) | 5 |
DEPC water | 10 |
Total volume | 30 |