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Table 4 Real Time PCR reaction mixture

From: Biochemical and molecular responses of Rosa damascena mill. cv. Kashan to salicylic acid under salinity stress

Compound Amount (μL)
cDNA (1:10 diluted) 6
Siber Green qPCR Mix 4
Forward primer (10 μM) 5
Reverse primer (10 μM) 5
DEPC water 10
Total volume 30