Fig. 8
From: Involvement of MdWRKY40 in the defense of mycorrhizal apple against fusarium solani
MdWRKY40 binds to the MdGLU promoter. (A) MdWRKY40 was divided into two fragments, N (N) terminus, and C (C) terminus. (B) The optimal concentration of 3-AT was determined by cloning proMdGLU into the pHIS2 vector. (C) MdWRKY40 interacted with MdGLU promoter fragments as per the Y1H assay. (D) An EMSA analysis revealed that MdWRKY40 binds to the W-box II of the MdGLU promoter. (E) Luciferase reporter (LUC) assays showed the MdWRKY40-mediated activation of proMdGLU. EMSA, electrophoretic mobility shift assay. *P < 0.05. **P < 0.01