Fig. 6
From: Suppression of GhGLU19 encoding β-1,3-glucanase promotes seed germination in cotton
ABA biosynthesis was deregulated in GhGLU19-Ass. A Expression heatmap of genes related to MVA and ABA biosynthesis. B Transcript levels of MVA pathway and ABA biosynthesis related genes in transgenic lines and W0 by qRT-PCR analysis. Genes encoding key rate-limiting enzymes in MVA and ABA biosynthesis were involved in HMGCS, MVK, MVAK, FDPS and ABA2. Their expression levels were significantly evaluated in 8 h imbibed seeds of GhGLU19-overexpressing transgenic lines compared to W0. Relative expression was calculated using Histone3 as an internal reference. Three independent biological replicates were analyzed with three technical repeats. Vertical bars represent means ± SD. * p < 0.05. ** p < 0.01. C Endogenous ABA content of imbibed seeds at 2 h and 8 h were measured by high performance liquid chromatography with tandem mass spectrometry among transgenic lines and W0. Three independent biological replicates were analyzed. Vertical bars represent means ± SD. * p < 0.05. ** p < 0.01