Fig. 1
From: Suppression of GhGLU19 encoding β-1,3-glucanase promotes seed germination in cotton
The expression profile and enzyme activity of GhGLU19. A The expression profiling of GhGLU19A (Gh_A04G0109) and GhGLU19D (Gh_D05G3612) in tissues of G. hirsutum acc. TM-1. Imbibed seeds at 0, 5, 10, 24 h, root, stem, leaf, petal, stamen, pistil, calycle, flower, and ovules at 0, 5, 10 and 20 days post anthesis (DPA) were used for transcriptional analysis. The FPKM were used to calculate the expression level of GhGLU19A and GhGLU19D, respectively. Colored squares indicated expression levels from 0 (blue) to 20 (red). B The expression level of GhGLU19 in imbibition seeds at 2, 4, 8, 12, 24 and 48 h after imbibition compared with that at 0 h by qRT-PCR analysis. Three independent biological replicates were analyzed with three technical repeats. Student’s t-test, * P < 0.05, ** P < 0.01. C Enzyme activity of GhGLU19 was detected by the spectrophotometric method, and the activity was calculated by measuring the content of glucose. The pET-30a plasmid was used as control