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Fig. 6 | BMC Plant Biology

Fig. 6

From: Identification and characterization of a new geminivirus from soybean plants and determination of V2 as a pathogenicity factor and silencing suppressor

Fig. 6

SGVA V2 functions as a pathogenicity determinant. A SGVA-ZZV2-STOP and SGVA-ZZV2-3738AA was constructed as shown in the schematic diagram. Symptoms of N. benthamiana plants inoculated with SGVA-ZZ, SGVA-ZZV2-STOP and SGVA-ZZV2-3738AA were photographed at 14 dpi and 21 dpi, respectively (B, E). The accumulation of SGVA-ZZ was detected by western blotting (C, F) and quantitative reverse transcription PCR (qRT-PCR) (D, G) respectively. Coomassie brilliant blue-stained RuBisCo large subunit protein (CBB) was used to show sample loadings. The expression of NbUBC was used as an internal control in qRT-PCR. The results are presented as means ± SD from three biological replicates per treatment. Bars represents the mean ± standard deviation (SD). The statistical significance between treatments was determined using Duncan's multiple range test (p* < 0 .05, p** < 0.01)

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