Fig. 5

Suppression of RNA silencing by SGVA V2. A The leaves of GFP-transgenic 16C line were co-infiltrated with agrobacterium suspension harboring 35S-GFP expressing GFP and the recombinant vectors expressing SGVA V2 protein as indicated. The leaves expressing pGD + GFP were used as negative control and leaves expressing P19 + GFP were used as positive control. The photographs were taken under UV light at 3 d post infiltration. B Western blotting analysis of GFP accumulation in the co-infiltrated leaf patches at 3 d post infiltration. Coomassie brilliant blue-stained RuBisCo large subunit protein (CBB) was used to show sample loadings. C qRT-PCR analysis of GFP accumulation in the co-infiltrated leaf patches at 3 d post infiltration. The expression of NbUBC was used as an internal control in qRT-PCR. The results are presented as means ± SD from three biological replicates per treatment. Bars represents the mean ± standard deviation (SD). The statistical significance between treatments was determined using Duncan's multiple range test (p* < 0 .05, p** < 0.01)