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Fig. 3 | BMC Plant Biology

Fig. 3

From: Identification and characterization of a new geminivirus from soybean plants and determination of V2 as a pathogenicity factor and silencing suppressor

Fig. 3

Systemic infection of SGVA-ZZ in Nicotiana benthamiana. A Construction of infectious cDNA clone. Whole viral genome was cloned using fragments F1 (2762 bp) and F2 (2377) digested with BglII/HindIII and HindIII/SalI, respectively and sequentially ligated into pGD vector to obtain the recombinant clone PGDSGVA-ZZ. B Systemic symptoms induced by SGVA-ZZ in N. benthamiana plants were observed at 13 dpi and 23 dpi, respectively. C The presence of SGVA-ZZ in N. benthamiana systemic leaves was confirmed (13 dpi) by PCR using primers specially targeting SGVA-ZZ CP. D SGVA accumulation in N. benthamiana systemic leaves was detected by conducting western blotting analysis with anti-SGVA CP antibodies. Coomassie Brilliant Blue (CBB) staining of the large subunit of RuBisCo served as a loading control

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