Fig. 6

WGCNA during the interconversion between CH and CL flowers of V. prionantha. (A) Dendrograms of all DEGs are clustered based on a dissimilarity measure (1-TOM). All leaves in the tree represent a single gene, and the major tree branches constitute five distinct modules (M1–M5) shown in different colors. (B) Heatmap of the module–trait relationships. The trait refers to the structural changes during interconversion between chasmogamous and cleistogamous flowers. The top color panel represents Pearson’s correlation coefficient, with red for positive correlation and blue for negative correlation. Each cell contains the corresponding correlation and p-value. (C, D) GO enrichment analysis of module1 (C) and module2 (D). In each analysis, the top 10 GO terms are shown. P-values represent the significance of enrichment. Circles indicate the target genes, and the sizes are proportional to the number of DEGs. (E, F) The top 20 genes ranked by the Maximal Clique Centrality (MCC) score identified in module1 (E) and module2 (F). Nodes represent hub genes and are labeled on circles, and co-expressed genes are linked by dashed gray lines. (G) GIGANTEA (Vp8612:GI) gene co-expression network analysis. All co-expression genes (Mutual-Rank < 10) are labeled in red hexagons. (H) Expression profile of the co-expressed genes with Vp8612:GI. Heatmap displays the expression in transcripts per million (TPM) (left) and fold change (CL/CH) (right) in each floral stage