Fig. 2
Effect of individual and combined stress response on rice plants. A Level of superoxide radicles in drought, pathogen and combined stress. After two days of plants reaching to severe stress, leaves were stained with NBT. B Levels of H2O2 in drought, pathogen and combined stress was quantified using DAB staining. C Photographs showing Evan’s blue staining to measure membrane stability. D Quantification of Evan’s blue dye accumulation. Minimum five biological replicates were used for quantification. Graphs showing mean values ± SE. Significant differences were determined at p < 0.0001 (estimated by one-way ANOVA using Tukey’s HSD analysis