Table 1 Detailed information of the newly identified SbALDH members including their subfamilies, structural arrangements, and subcellular localization
Family | Locus ID | Transcript ID | Annotation | Coordinate (5’ to 3’) | Length | Conserved domain/sites | MW (kDa) | pI | Localization | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Transcript (nt) | CDS (nt) | Protein (aa) | PF00171 start-stop | PS00687 yes/no | PS00070 yes/no | ||||||||
2 | Sobic.004G250900 | Sobic.004G250900.2 | SbALDH2B1a | 59,721,748–59,725,595 | 2967 | 1272 | 423 | 1–413 | Yes | Yes | 45.67 | 6.38 | Mitochondrion |
Sobic.004G250900.3 | SbALDH2B1b | 59,720,135–59,725,595 | 3507 | 1656 | 551 | 79–541 | Yes | Yes | 58.79 | 6.65 | Mitochondrion | ||
Sobic.004G250900.4 | SbALDH2B1c | 59,720,879–59,725,595 | 3246 | 1656 | 551 | 79–541 | Yes | Yes | 58.79 | 6.65 | Mitochondrion | ||
Sobic.010G113000 | Sobic.010G113000.1 | SbALDH2B2a | 11,756,541–11,766,808 | 2429 | 1671 | 556 | 84–546 | Yes | Yes | 60.09 | 7.20 | Mitochondrion | |
Sobic.010G113000.2 | SbALDH2B2b | 11,759,710–11,766,808 | 2630 | 1644 | 547 | 75–537 | Yes | Yes | 59.21 | 6.65 | Mitochondrion | ||
Sobic.003G203500 | Sobic.003G203500.1 | SbALDH2C1a | 53,331,225–53,339,384 | 1971 | 1506 | 501 | 29–491 | Yes | Yes | 54.33 | 5.99 | Chloroplast | |
Sobic.003G203500.2 | SbALDH2C1b | 53,331,225–53,339,384 | 2060 | 1272 | 423 | 29–416 | Yes | Yes | 45.73 | 5.84 | Chloroplast | ||
Sobic.003G203600 | Sobic.003G203600.1 | SbALDH2C2a | 53,368,965–53,376,601 | 2043 | 1515 | 504 | 32–494 | Yes | Yes | 54.24 | 5.47 | Chloroplast | |
Sobic.003G203600.2 | SbALDH2C2b | 53,368,965–53,376,625 | 2264 | 1272 | 423 | 1–413 | Yes | Yes | 45.70 | 5.34 | Chloroplast | ||
Sobic.003G203600.3 | SbALDH2C2c | 53,374,514–53,376,601 | 1570 | 1245 | 414 | 1–404 | Yes | Yes | 44.65 | 5.23 | Chloroplast | ||
Sobic.010G178300 | Sobic.010G178300.1 | SbALDH2C3 | 51,625,698–51,631,645 | 2164 | 1566 | 521 | 45–511 | Yes | Yes | 56.26 | 5.78 | Chloroplast | |
3 | Sobic.004G300800 | Sobic.004G300800.1 | SbALDH3E1 | 63,955,597–63,960,188 | 1971 | 1461 | 486 | 1–442 | Yes | Yes | 54.19 | 6.59 | Chloroplast |
Sobic.006G163300 | Sobic.006G163300.2 | SbALDH3E2a | 52,091,479–52,098,020 | 2119 | 1491 | 496 | 24–452 | Yes | Yes | 54.56 | 8.64 | Chloroplast | |
Sobic.006G163300.3 | SbALDH3E2b | 52,091,484–52,097,728 | 1741 | 1491 | 496 | 24–452 | Yes | Yes | 54.56 | 8.64 | Chloroplast | ||
Sobic.005G064800 | Sobic.005G064800.1 | SbALDH3H1 | 7,268,869–7,273,754 | 2377 | 1437 | 478 | 1–431 | Yes | Yes | 52.05 | 8.07 | Chloroplast | |
Sobic.008G057500 | Sobic.008G057500.1 | SbALDH3H2 | 5,994,822–6,001,133 | 1889 | 1464 | 487 | 1–437 | Yes | Yes | 52.42 | 9.45 | Chloroplast | |
5 | Sobic.004G058600 | Sobic.004G058600.1 | SbALDH5F1a | 4,685,051–4,695,810 | 2412 | 1584 | 527 | 62–521 | Yes | Yes | 56.01 | 8.31 | Mitochondrion |
Sobic.004G058600.2 | SbALDH5F1b | 4,685,097–4,695,810 | 2075 | 1551 | 516 | 62–494 | Yes | Yes | 54.92 | 8.25 | Mitochondrion | ||
Sobic.004G058600.3 | SbALDH5F1c | 4,685,092–4,695,810 | 2261 | 1482 | 493 | 28–487 | Yes | Yes | 52.40 | 6.22 | Mitochondrion | ||
Sobic.004G058600.4 | SbALDH5F1d | 4,687,581–4,695,576 | 1953 | 1176 | 391 | 1–385 | Yes | Yes | 41.50 | 5.70 | Mitochondrion | ||
Sobic.004G058600.5 | SbALDH5F1e | 4,685,097–4,695,810 | 2108 | 1584 | 527 | 62–521 | Yes | Yes | 56.01 | 8.31 | Mitochondrion | ||
6 | Sobic.002G062500 | Sobic.002G062500.1 | SbALDH6B1 | 6,035,846–6,043,359 | 2684 | 1623 | 540 | 55–519 | No | Yes | 57.84 | 5.89 | Mitochondrion |
7 | Sobic.002G215700 | Sobic.002G215700.1 | SbALDH7B1 | 60,779,264–60,786,533 | 2031 | 1530 | 509 | 30–492 | Yes | Yes | 54.40 | 6.06 | Mitochondrion |
10 | Sobic.006G109500 | Sobic.006G109500.1 | SbALDH10A1 | 47,850,314–47,855,384 | 2070 | 1521 | 506 | 18–488 | Yes | Yes | 55.06 | 5.94 | Chloroplast, Mitochondrion, Peroxisome |
Sobic.007G130800 | Sobic.007G130800.1 | SbALDH10A2a | 54,746,028–54,751,628 | 1731 | 1518 | 505 | 18–487 | Yes | Yes | 54.91 | 5.16 | ||
Sobic.007G130800.2 | SbALDH10A2b | 54,745,913–54,751,628 | 1976 | 1182 | 393 | 1–375 | Yes | Yes | 42.75 | 4.85 | |||
11 | Sobic.007G140700 | Sobic.007G140700.1 | SbALDH11A1 | 56,977,075–56,981,706 | 2088 | 1497 | 498 | 26–486 | Yes | Yes | 53.28 | 6.80 | Cytoplasm |
12 | Sobic.009G212600 | Sobic.009G212600.1 | SbALDH12A1 | 55,816,131–55,822,788 | 2152 | 1650 | 549 | 55–510 | Yes | Yes | 60.58 | 6.90 | Mitochondrion |
18 | Sobic.003G356000 | Sobic.003G356000.1 | SbALDH18B1 | 67,427,644–67,434,548 | 2783 | 2190 | 729 | 295–584 | No | Yes | 78.36 | 5.99 | Cytoplasm |
Sobic.009G160100 | Sobic.009G160100.1 | SbALDH18B2a | 51,779,954–51,800,133 | 2804 | 2151 | 716 | 288–570 | No | Yes | 77.71 | 5.92 | Cytoplasm | |
Sobic.009G160100.2 | SbALDH18B2b | 51,779,954–51,800,133 | 2800 | 2151 | 716 | 288–570 | No | Yes | 77.71 | 5.92 | Cytoplasm | ||
Sobic.009G160100.3 | SbALDH18B2c | 51,779,375–51,787,129 | 3460 | 2151 | 716 | 288–570 | No | Yes | 77.71 | 5.92 | Cytoplasm | ||
Sobic.009G160100.4 | SbALDH18B2d | 51,779,375–51,787,112 | 3447 | 2151 | 716 | 288–570 | No | Yes | 77.71 | 5.92 | Cytoplasm | ||
22 | Sobic.002G426100 | Sobic.002G426100.1 | SbALDH22A1 | 77,251,658–77,257,367 | 2414 | 1782 | 593 | 52–521 | Yes | Yes | 65.45 | 7.48 | Chloroplast |