Fig. 3

Phenotype investigation and degree of membrane damage between WT and OE. Six-week-old tomatoes were used to detect physiological indexes related to cold stress. (a). Expression level of VaPYL9 in grape under 4 ℃ stress for 0 h, 6 h, 12 h, 24 h, 48 h. (b). Expression level of VaPYL9 in WT and transgenic tomatoes under cold stress and nonstress. (c). Tomatoes phenotype was observed in WT and OE under 4 ℃ for 0 h, 48 h. (d). The determination of relative electrolyte leakage in WT and OE plants under 4 ℃ for 0 h, 6 h, 12 h, 24 h, 48 h. (e). The DAB and NBT staining of WT and OE plants under 4 ℃ for 0 h, 12 h, 48 h. (f). The content of H₂O₂ was determined in WT and OE plants under 4 ℃ for 0 h, 6 h, 12 h, 24 h, 48 h. (g). The content of MDA was determined in WT and OE plants under 4 ℃ for 0 h, 6 h, 12 h, 24 h, 48 h. (h). The content of proline was determined in WT and OE plants under 4 ℃ for 0 h, 6 h, 12 h, 24 h, 48 h. The bar represents the value of standard error (p < 0.01). Lowercase letters represent the significance level. Means and SE values were calculated from at least three independent experiments