Fig. 2
From: MdNup62 interactions with MdHSFs involved in flowering and heat-stress tolerance in apple
Identification and analysis of MdNup62. a Phylogenetic analysis of Rosaceae Nup62. b The conservative domain of Rosaceae Nup62. c Subcellular localization of MdNup62. The upper panel shows 35S::EGFP, and the lower panel shows 35S::MdNup62-EGFP. d and e Analyses of MdNup62 expression levels in diverse ‘Nagafu No. 2’ apple tissues d and in different flower bud developmental stages of ‘Nagafu No. 2’ e. (f) The phenotype of ‘Nagafu No. 2’ tissue-cultured seedlings (upper panels) and the in situ accumulation of superoxide radical (O2−) at 0, 1, 3, and 6 h under heat treatment conditions (lower panels). Bar = 1 cm. g MdNup62 expression levels in ‘Nagafu No. 2’ tissue-cultured seedling leaves at 0, 1, 3, and 6 h under heat-treatment conditions. Each sample was analysed with three biological replicates, each comprising three technical replicates. Means followed by different lowercase letters are significantly different at the 0.05 level. The same below