Skip to main content

Table 2 Profiles of gene (qRT-PCR) and protein levels (TMT data) involved in flavonoid and lipid synthesis in sea buckthorn pulp at different developmental stages

From: Identification of the key flavonoid and lipid synthesis proteins in the pulp of two sea buckthorn cultivars at different developmental stages

Protein name Definition Protein level (TMT data) Gene level (qRT-PCR)
XE30/SJ30 XE50/SJ50 XE70/SJ70 XE30/SJ30 XE50/SJ50 XE70/SJ70
CHS chalcone synthase [EC:2.3.1.74] 1.87a 1.29 0.99 17.32a 4.99a 5.13a
F3H flavanone 3-hydroxylase [EC:1.14.11.9] 1.55a 1.71a 0.95 14.82a 11.39a 0.77
fabD [acyl-carrier-protein] S-malonyltransferase [EC:2.3.1.39] 0.78 1.20 1.58a 0.82 2.47a 1.11
FATA fatty acyl-ACP thioesterase A [EC:3.1.2.14] 0.65a 1.07 1.59a 2.37 2.21 1.11
FAB2 acyl-[acyl-carrier-protein] desaturase [EC:1.14.19.2; 1.14.19.11; 1.14.19.26] 0.78 0.95 1.78 1.64 1.71 1.00
GPAT glycerol-3-phosphate acyltransferase [EC:2.3.1.15] 1.35 1.74a 1.17 1.11 3.67a 1.29
LPIN phosphatidate phosphatase LPIN [EC:3.1.3.4] 0.98 0.85 1.50a 1.47 1.94a 1.12
MGD 1,2-diacylglycerol 3-beta-galactosyltransferase [EC:2.4.1.46] 1.18 0.45a 0.49a 2.87a 0.87 1.32
plcC phospholipase C [EC:3.1.4.3] 1.09 1.48a 1.90a 1.76a 5.15a 0.90
  1. qRT-PCR analysis of cDNA isolated from developing berries at 30, 50 and 70 DAF. The actin gene was used as an internal standard. Values represent the means of three biological triplicates. aindicates a significant difference at 0.05 level