Fig. 1

Characterization of TuRLK1 in PI428309 and G1812. a RNA-seq data showed that the transcript level of TuRLK1 in PI428309 was significantly induced after Pst CY33 infection. FPKM: Fragments Per Kilobase of exon model per Million mapped fragments. hpi: hours post inoculation. b Transcript levels of TuRLK1 in PI428309 were examined by qRT-PCR, using ACTIN as an internal control. Leaves at the seedling stage were detached for RNA isolation and qRT-PCR after inoculation with Pst CY33 at the indicated time points. Error bars represent the standard deviation of 3 independent biological replicates. c Protein sequences alignment between TuRLK1 in PI428309 and G1812. The protein sequences shaded in blue are identical. Domains in the protein sequences were predicted according to the SMART program. LRR motifs are in the red or blue rectangular blocks. Red line indicates the kinase domain, and green line highlights the transmembrane region. d Phylogenetic tree analysis with TuRLK1 and 20 other RLKs. The phylogenetic tree, constructed according to the protein sequences of the 21 RLKs under the neighbor-joining method, was depicted using MEGA5.0 software