Fig. 6

TaSL1 regulated both spike and kernel length and the distribution of its preferred haplotype. A Gene marker BJ-P2010K and BJ-P2010 was developed based on a 416 bp deletion and a SNP in the promoter of TraesCS7A02G205100, respectively. The solid black box represents exons and the solid black line represents introns; The gray box represents 5′-UTR and the gray arrow box represents 3′-UTR; The white box and dotted line represents promoter. B Gene marker BJ-6840 was developed based on a 113 bp deletion in fist intron of TraesCS7A02G200000. C Agarose gel plots of gene markers. Upper left picture shows marker BJ-6840; Bottom left picture shows marker BJ-P2010K; Right picture shows the digestion of PCR product of primer set BJ-P2010-F/R with enzyme SmaI. The plots of different markers were from different gels. The completed gels are shown in Figure S6. M, molecular marker; CS, genotype of Chinese spring; Z, genotype of Zhou8425B, H, genotype of heterozygous. D Gene markers BJ-P2010K, BJ-P2010 and BJ-6840 were associated with both spike length and kernel length in 265 wheat landraces. E Distributions of two haplotypes of TaSL1(represented by BJ-P2010) in 429 cultivars population in different ecological regions of China. I, North winter wheat zone; II, Huanghuai winter wheat zone; III, The Yangtze river winter wheat zone; IV, Southwest winter wheat zone; V, South China winter wheat zone; VI, Northeast spring wheat zone; VII, North spring wheat zone; VIII, Northwest spring wheat region; IX, Qinghai-Tibet Spring-Winter Wheat zone; X, Xinjiang winter-spring wheat zone. F Distributions of two haplotypes of TaSL1 in 334 varieties from the six worldwide wheat production regions (Excluding China). The relative size of the pie chart indicates the relative size of the sample