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Fig. 5 | BMC Plant Biology

Fig. 5

From: Combining QTL mapping and gene co-expression network analysis for prediction of candidate genes and molecular network related to yield in wheat

Fig. 5

Expression analysis of candidate genes between parent Chinese Spring and Zhou8425B. A The relationship between gene expression level and traits variation. The gene expression level was reflected by RPKM value. The green dot indicates Chinese Spring and the blue dot indicates Zhou8425B. The gene significance (GS) was defined as mediated P-value of each gene in the linear regression between gene expression and the traits. *, Statistical test P-value is significant at the 0.05 level. **, Statistical test P-value is significant at the 0.01 level. SL, spike length (cm); KL, kernel length (mm); KW, kernel width (mm); TKW, thousand kernel weight (g); GR, germination ratio (%); GI, weighted germination index (%). B Using qRT-PCR to verify the expression levels of candidate genes between parent Chinese Spring and Zhou8425B. CS, Chinese Spring; Z, Zhou8425B. Total RNA was extracted from 21 DPA wheat spike. Gene expression was calculated using the 2-ΔΔCt methods with TaActin as an internal control. Data are means (±SE) of three biological replicates, n = 3

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BMC Plant Biology

ISSN: 1471-2229

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