Fig. 3
From: Discovery of a novel mitochondrial DNA molecule associated with tetrad pollen sterility in potato
Stoichiometric differences between P-3 and the 859-bp band. Electrophoresed gels of the PCR-amplified bands via (a) rpl5rps14outF/nad6 and (b) rpl5rps14outF/ALM_5 (for the 859-bp-band) primer sets. 1, cv. Alwara; 2, 18H225; 3, 15H156; 4, 19H69; 5, 19H77; 6, 19H72-1; 7–9, S. stoloniferum PI 498030, PI 545726 and PI 558455, respectively; 10, 17H117-9; 11, 17H131; 12, 10H6-1; 13, 17H125-1. A 1 kb Plus DNA ladder (Invitrogen™) was used as a size marker in the first lane. Full-length gels are presented in Supplementary Fig. S4. c–e Amplification differences detected via real-time PCR. Relative quantities compared with those of aprt (adenine phosphoribosyl transferase, a housekeeping nuclear gene; Nicot et al. [41]) were measured for rpl5-nad6 (c) and rpl5-rps10 (d), and their differences are shown as the relative quantities of rpl5-rps10 divided by those of rpl5-nad6 (e). 1–5, S. stoloniferum PI 497994, PI 498038, PI 545780, PI 558450 and PI 558455, respectively; 6, 15H156; 7, 17H120-1; 8, 18H225; 9, 17H118-3; 10, 17H117-9; 11, 17H131; 12 and 13, S. verrucosum PI 275260 and PI 498061, respectively; 14, cv. Alwara; 15, cv. Désirée. The samples with TSCsto are underlined