Fig. 2

Validation of the homologous recombination mediated by different pair repeats. A Schematic of the primer design of direct repeats that could mediate the homologous recombination in the representative circular molecule. B Schematic of the experimental design following the exchange of reverse primers. C PCR production of validation results. From left to right, each of the five lanes represents a set of three experiments, namely, repeat1, repeat2, and repeat3. Each set of experiments is, from left to right, marker, major conformation 1, major conformation 2, alternative conformation 1, and alternative conformation 2