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Fig. 7 | BMC Plant Biology

Fig. 7

From: Overexpression of grape ABA receptor gene VaPYL4 enhances tolerance to multiple abiotic stresses in Arabidopsis

Fig. 7

Overexpression of VaPYL4 improves plant performance after multi-stress treatment. A Phenotypes of WT and OE plants during the treatment with different abiotic stresses. Three-week-old seedlings were first watered with 100 mM NaCl solution for 5 days and then treated at 4℃ for 3 days, followed by a drought treatment by withholding water for 14 days and a 3-day recovery period. Representative images were taken before treatment (stage 1), at 5 days (stage 2) of drought treatment, and at the end of the treatment (stage 3), respectively. The damages observed in WT leaves at stage 2 were indicated by white arrows. Scale bars: 1 cm. B Images showing the development of seedlings and siliques after multi-stress treatment. Photographs were taken at 50 days post-germination. Scale bar: 1 cm. C Survival rates of WT and OE plants. Data are collected from three replicates, and each replicate consists of at least 45 seedlings. D Flowering time of WT and OE plants treated with or without abiotic stresses. The plants with at least one flower were considered to be at the flowering stage. Data are collected from three replicates, and each replicate consists of at least 10 and 50 seedlings for control and multi-stress treatment, respectively. The number of plants survived from treatment and used for flowering time record is shown above the figure. EF Fresh weight of seedling (E) and siliques (F). The individual plants without roots were used for the measurement of fresh weight (FW). For the measurement of FW of siliques, all the siliques from the same genotype were pooled and a number of 10 siliques were collected as a sample. The replicates from three experiments are shown above the bars. Data are means ± SD. **P < 0.01

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