Fig. 3
VaPYL4-overexpressing (OE) plants are hypersensitive to exogenous abscisic acid (ABA). A Schematic illustration of T-DNA region of the pSAK277-VaPYL4 vector for gene overexpression. CaMV35S, cauliflower mosaic virus 35S promoter; TNOS, terminator of nopaline synthase gene; OCS, octopine synthase terminator; PNOS, promoter of nopaline synthase gene; RB, right border; LB, left border. B Seeds germination on 1/2 MS medium containing 0.3 µM ABA. After incubation at 4℃ for 2 d, wild-type (WT) and OE seeds were sown on 1/2 MS medium containing 0.3 µM ABA. The emergence of radicle was measured as germination, and the germination rates were calculated at 3 d after sowing. Scale bars correspond to 0.5 cm. C Cotyledon greening of WT and OE plants on 1/2 MS plates for 6 d. Scale bars correspond to 0.5 cm. Data are collected from three replicates and shown as averages of around 100 seeds. Date are presented as means ± SD. **P < 0.01