Fig. 7

Effect of the repeated wounding on the growth of the seedlings with the IBU application or P2K1 mutation, or the combination of P2K1 mutation and IBU application. a Leaf area of WT seedlings. b Leaf area of p2k1-3 mutant seedlings. The first wounding, the first IBU application, or first wounding plus IBU application were performed at 21th day of growth, indicated by the red line. c Root length. d Fresh weight. e Dry weight. WT-control: the un-wounded WT seedlings, WT-wounded: the wounded WT seedlings, WT-wounded plus IBU: the wounded WT seedlings treated with IBU, p2k1-3-control: the un-wounded p2k1-3 mutant seedling, p2k1-3-wounded: the wounded p2k1-3 mutant seedlings, and p2k1-3-wounded plus IBU: the wounded p2k1-3 mutant seedlings treated with IBU. The values represent means ± SD from sixteen independent seedlings. For Fig. 7a-b, different letters on top of the bars indicate significant differences (P < 0 .05) among the different treatments at the same time. For Fig. 7c-e, the means denoted by the same letter did not significantly differ at P < 0.05 among the different treatment