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Table 2 Mean of inhibition zones (diameter in millimeters) after in vitro exposure of isolates to A. indica methanol extract in different concentrations

From: In-vitro and in-silico antibacterial activity of Azadirachta indica (Neem), methanolic extract, and identification of Beta.d-Mannofuranoside as a promising antibacterial agent

Methanolic extract concentrations (%)

Bacterial isolates

50

25

12.5

6.25

3.125

1.5

E. coli (n = 70)

17.5 ± 1

15.5 ± 1

13.5 ± 0.5

12 ± 0.8

10 ± 0.5

N.A

K. pneumoniae (n = 9)

19 ± 1.6

17 ± 2

15.4 ± 2

13.5 ± 1.8

11 ± 1.9

N.A

Citrobacter spp. (n = 1)

22 ± 1

20 ± 1.5

18 ± 0.5

16 ± 1.1

11 ± 0.8

N.A

E. faecalis (n = 8)

16.5 ± 1.8

14.5 ± 2.2

13 ± 2.1

9 ± 1.6

8 ± 1.9

N.A

P. aeruginosa (n = 5)

21 ± 1

19 ± 0.8

17 ± 1.3

16 ± 1.5

13 ± 1.8

N.A

Proteus spp. (n = 5)

17.5 ± 1.8

15 ± 1.6

11 ± 1.2

9 ± 0.9

8 ± 0.9

N.A

S. epidermidis (n = 1)

15 ± 1.1

14 ± 1.5

13 ± 1.2

12 ± 0.5

8 ± 0.5

N.A

S. aureus (n = 1)

13 ± 0.5

11 ± 0.8

10 ± 0.7

9 ± 0.4

8 ± 0.5

N.A

E. coli ATCC® 25,922

18 ± 0.4

16 ± 1

14 ± 1

12.5 ± 0.4

8 ± 1

NA

S. aureus ATCC® 25,923

22 ± 0.8

20 ± 1.7

18 ± 2

15 ± 2

10 ± 0.5

NA

  1. Diameters of inhibition zones were measured in millimeters. Less than 9 mm zone was considered inactive, 9–12 mm as partially active, 13–18 mm as active, and 18 mm were very active. N.A = Not active