Fig. 4

Analysis of L. regale scales after transient expressing the LrWRKY3 RNAi vector. a The symptoms of L. regale scale after F. oxysporum inoculation, in which the LrWRKY3 RNAi vector and the empty RNAi vector was expressed, respectively. b Measurements of scales lesions caused by F. oxysporum infection. The results were shown as the average values calculated from three replicates and the Student’s t test was used to analyze the statistical difference (** p < 0.01). c The expression level of LrWRKY3 in L. regale scale transiently expressing the LrWRKY3 RNAi construct was evaluated by qRT-PCR. The empty RNAi vector infected samples with F. oxysporum inoculation was the control of statistical analysis. The results were calculated by the 2^-ΔΔCt method and analyzed by the Student’s t test (** p < 0.01, * p < 0.05)