Fig. 2
Gene expression and resistance analyses of LrWRKY3 T2 generation transgenic tobacco lines. a The expression level of LrWRKY3 in T2 generation transgenic tobacco by qRT-PCR. The LrWRKY3 was stably expressed in transgenic tobacco lines. WT: wild-type tobacco; W3–1/2/3/6/19/20/27/34/35/36/44: LrWRKY3 T2 transgenic tobacco lines. b The root inoculation assay revealed the enhanced resistance of four LrWRKY3 T2 transgenic tobacco lines (W3–6/20/35/44) against F. oxysporum. c The leaf inoculation assay revealed the enhanced resistance of four LrWRKY3 T2 transgenic tobacco lines (W3–6/20/35/44) against F. oxysporum. d The results of leaf inoculation showed that the lesion areas in transgenic tobacco were significantly smaller than that in the WT. The results were shown as average values calculated from three replicates and calculated by the 2-ΔΔCt method and analyzed by the Student’s t test (** p < 0.01)