Fig. 5
DcRAD51D and DcPS1 physically interacts with OSDLa. A Y2H assay for the interaction of DcRAD51D and OSDLa. Co-transformation with pGADT7-T and pGBKT7-Lam was used as a negative control, while co-transformation with pGADT7-T and pGBKT7–53 was used as a positive control. B BiFC analysis for in vivo interaction between DcRAD51D and OSDLa. C BiFC analysis for in vivo interaction between DcPS1 and OSDLa. D DcPS1 were unable to associate with DcRAD51D. For two combinations, DcRAD51D-YFPN + YFPC  (Co-transformation with DcRAD51D-YFPN and YFPC) (E), YFPN + OSDLa-YFPC  (Co-transformation with YFPN and OSDLa-YFPC) (F) and DcPS1-YFPN + YFPC  (Co-transformation with DcPS1-YFPN and YFPC) (G) were a negative control. Y, YFP; Chl, Chlorophyll; BF, Bright Field; M, Merge