Fig. 6From: Degradome sequencing reveals an integrative miRNA-mediated gene interaction network regulating rice seed vigorPhylogenetic analysis, subcellular localization and yeast two-hybrid (Y2H) assays of Os02g0817500. A Phylogenetic tree. XP_015626381.1, outlined with a red box represents Os02g0817500. B Subcellular localization of Os02g0817500, yellow-green indicated that Os02g0817500 expressed in the cytoplasm. C Nuclear system and (D) membrane system Y2H assays. The interaction partners of Os02g0817500 were identified by growing yeast on SD/−Leu/−Trp/−His/−Ade/X-α-gal and SD/−Leu/−Trp/−His/−Ade/X-gal plates. The pGADT7 and pPR3-N empty vectors were used as negative controls in the nuclear system and membrane system Y2H assays, respectivelyBack to article page