Fig. 1

Characterization of unaged and artificially aged seeds of different genotypes of rice. A Seed germination rate; B RT-qPCR analysis of the expression level of miR164c. Data represent mean ± standard deviation (SD; n = 3). Significant differences among samples were determined using Student’s t-test (*P < 0.05, **P < 0.01, ***P < 0.001). In (B), The expression level of miR164c in unaged WT seeds was set as 1. WT indicates the wild-type rice cultivar ‘Kasalath’; MIM164c and OE164c indicate two modified lines in ‘Kasalath’ background, miR164c-silenced line ‘L13–1–2-1’ and miR164c overexpression line ‘L4–1–3-1’, respectively