Fig. 7

Expression patterns of the randomly selected 12 miRNAs (line charts) and their 15 target genes (bar charts) in susceptible ROC22 and resistant FN39 infected by Sorghum mosaic virus through qRT-PCR analysis. A The miRNAs and their target genes were all up-regulated in both varieties. B The miRNAs and their target genes were all down-regulated in both varieties. C The miRNAs and their target genes showed inconsistent expression patterns in susceptible and resistant varieties. CK and SrMV represent the virus-free sample (control group) and SrMV-infected plants with mosaic symptoms (treatment group), respectively. The right and left y axis represent the relative expression of miRNA and its predicted target gene, respectively. Each bar represents the mean values of three replicates ± standard error (n = 3). Different lowercase letters indicate a significant difference, as determined by the least-significant difference test (p-value < 0.05). rbcLBP, Rubisco large subunit-binding protein; UCH, ubiquitin carboxyl-terminal hydrolase; RDDP, RNA dependent DNA polymerase; GRF2, growth-regulating factor 2; NFYC-1, nuclear transcription factor Y subunit C-1; CML10, calmodulin-like protein 10; PRPL2, 50S chloroplastic ribosomal protein L2; ARF6, auxin response factor 6; PP2Ac, phosphatase 2A-3 catalytic subunit-like; ATG8c, autophagy-related protein 8C-like; ELP6, elongator complex protein 6; PRR73, pseudo-response regulator 73; ATPs-3, ATP-sulfurylase 3; LAST-3, low affinity sulfate transporter 3-like; and PRPS6, 30S chloroplastic ribosomal protein S6