Fig. 6

Identification and stress treatments of PagNAC045 over-expressing transgenic tobacco lines. A RT-PCR validation of over-expressing transgenic lines with specific primers PagNAC045F1 and PagNAC045R1 (Supplementary Table S2); M, 2000 DNA marker; WT, wild type; T1-T6, transgenic lines; P, positive plasmid. B Biochemical analyses of superoxide dismutase (SOD), peroxidase (POD), malondialdehyde (MDA) and proline contents in the leaves of transgenic lines and WT. C Images of nitroblue tertazolium (NBT) staining. D Images of 3,3′-diaminobenzidine (DAB) staining. E Four-week-old tobacco plants in soil before the treatments (upper panels), and plants treated with 200 mM NaCl or 50 μM ABA for one week (bottom panels). Student’s t-test: t: *, P < 0.05; **, P < 0.01. Error bars indicate mean ± SD