Fig. 2

AHL9 is involved in dark-induced and ethylene-induced leaf senescence. A Phenotype of detached leaves from 3-week-old WT and two independent AHL9 overexpressing lines subjected to dark treatment. Detached leaves were incubated in MES buffer for 3 d under dark conditions. Scale bar = 1 cm. B Chlorophyll content of detached leaves from (A). The data were analyzed using one-way ANOVA analysis. Means with different letters above the bars indicate statistically significant results (P < 0.05). Data indicate means ± SD, n = 3. The experiment was conducted three times with similar results. C Phenotype of detached leaves from 3-week-old WT and two independent AHL9 overexpressing lines ACC or ACC + AOA in the dark. Detached leaves were treated with MES buffer, 100 µM ACC or 100 µM ACC + 500 µM AOA for 3 d under the dark conditions. D Chlorophyll contents in leaves from (C). The data were analyzed using one-way ANOVA analysis. Means with different letters above the bars indicate statistically significant results (P < 0.05). Data indicate means ± SD, n = 3. The experiment was conducted three times with similar results