Fig. 2

Transformation and molecular analysis of putative transgenic potato plants. a In vitro propagation of potato plants from culture of nodal segments on liquid MS medium, b No shoot regeneration from the cultured internodes on control selective MS medium containing antibiotics; c Complete direct shoot regeneration (100%) from non-inoculated internode pieces on control medium without antibiotic d Efficient direct shoot regeneration rate (35%) from inoculated internode segments with Agrobacterium harboring the constructs on the selective medium supplemented with antibiotics, e The young plants obtained from independent regeneration events, f PCR analysis of DNA isolated from transgenic plants with CaMV 35S forward primer (35S-F) and StAPI5 gene reverse specific primer (StAPI5-R). The expected band length is 754-bp. C: Negative control with non-transgenic potato plant (wild-type); P: positive control PCR reaction with plasmid as template; Lanes no. 1–10: DNA from independent transgenic potato plants and M: 1 kb DNA Ladder. g Southern blot analysis of the independent transgenic potato plants overexpressing StAPI5-OE gene. P: the pRI-AN201 binary vector containing the StAPI5 gene (positive control); lanes 1 and 2: independent transgenic potato plants; C: non-transformed potato (negative control); the arrows represent the random integration of transgene(s) into the host genome of transgenic potato plants