Fig. 5
From: MNB1 gene is involved in regulating the iron-deficiency stress response in Arabidopsis thaliana
qRT-PCR analysis of the key genes involved in Fe-deficiency stress. A-H qRT-PCR analysis of the key genes related to Fe uptake and translocation in the roots of Col and mnb1 lines. Col and mnb1 lines were grown vertically on MS agar plate for 10 days and then shifted to MS (+Fe, control) and Fe-deficient (−Fe) for 7 days. ACTIN8 was used as the internal control. Three independent repeated assays were conducted with similar results, each experiment with three replicates. Values are means and SD from three independent biological replicates. Statistically significant differences (Tukey’s test, p < 0.05) are marked by different lowercase letters. I IRT1 protein level under Fe-deficient stress. Col and mnb1 lines grown vertically on MS (+Fe) medium for 10 days and then shifted to MS (+Fe, control) and Fe-deficient (−Fe) for 7 days. Protein extracts from treated seedlings and analyzed by 10% SDS-PAGE and Western blot assay. Anti-IRT1 antibody (upper panel), Rubisco (lower panel) as control. Original images of full-length gels or blots (Additional file 4: Fig. S4)