Fig. 4
From: MNB1 gene is involved in regulating the iron-deficiency stress response in Arabidopsis thaliana
ROS accumulation in the mnb1 mutants and MNB1-overexpressing lines. A DAB staining of Col, mnb1, OE3, OE7 lines. Arabidopsis seedlings germinated on MS agar media for 2 weeks and then shifted to MS (+Fe, control) and Fe-deficient (−Fe) for 7 days. B Quantification of H2O2 from Col, mnb1-1, mnb1-2, OE3 and OE7 plants. Arabidopsis seedlings germinated on MS agar media for 2 weeks and then shifted to MS (+Fe, control) and Fe-deficient (−Fe) for 7 days, and seedling samples were obtained and quantified in H2O2 concentration. C The measurement of MDA in Col, mnb1-1, mnb1-2, OE3 and OE7 plants. Arabidopsis seedlings germinated on MS agar plates for 2 weeks and then shifted to MS (+Fe, control) or Fe-deficient (−Fe) for 7 days, and seedling samples were obtained and quantified in MDA concentration. Values are means and SD from three to four independent biological replicates. Statistically significant differences (Tukey’s test, p < 0.05) are marked by different lowercase letters