Table 4 Attributes of 12 microsatellite loci used for genotyping of Angelica dahurica and its cultivars
Locus | Forward and reverse primer sequences | Motifs | Product size range (bp) | Ta | GenBank Accession No. |
|---|---|---|---|---|---|
AD1 | F (FAM):TCCTCCAGCTGGCATAATAATAA R:ATTAAAAAGAACAAGGGGCTCAA | (TGC)6 | 111–123 | 55 | MH220032 |
AD7 | F (FAM):GCTCTCTTAAATTTCACCCCAAC | (ATTACC)4 | 131–155 | 55 | MH220035 |
R:TACTAGATTCTTCCAGAGCGACG | |||||
AD8 | F (TAMRA):TTCAACATGGTCATGTGAGTGAT | (GGAGTG)4 | 140–164 | 53 | MH220036 |
R:CCGTTGGAGGTCTTCTTGTAAAT | |||||
AD9 | F (TAMRA):CAACACACATGATCCAGAAGAAA R:GAGCTGGAGATAGTCTGTTGCAT | (TCTGCA)10 | 99–159 | 50 | MH220037 |
AD10 | F (FAM):AGACTGCACCTGTCTCATTTTTC | (GT)9 | 116–140 | 50 | MH220038 |
R:GGCTTGTAATTAATCTTTGCACC | |||||
AD11 | F (TAMRA):TTCGTCATTTAGAAACGATAGCA | (TCT)7 | 127–142 | 50 | MH220039 |
R:TCAATGGATACCACCACATCATA | |||||
AD14 | F (FAM):TGTACTCCATGGACTGGAGTCTT | (TCA)7 | 108–123 | 50 | MH220041 |
R:TTTGTTTTCTGACAAAGCCAAAT | |||||
AD17 | F (TAMRA):GGATCATGTTGATGATGGAAAAT | (AGA)7 | 145–163 | 50 | MH220042 |
R:TTCGATTACTACAGCAGATGAGC | |||||
AD19 | F (TAMRA):CCCCATTTCTCCCATAGATAGAT | (GCA)6 | 125–140 | 53 | MH220043 |
R:CCATTAATTGTTCTGCATTTTCC | |||||
AD22 | F (FAM): AAACAATATCAAATCAAATGGCG | (TTC)6 | 84–99 | 50 | MH844986 |
R: GTGGTGATGATGAATCTTGTGAA | |||||
AD23 | F (FAM): GCTTGACATATATCATCGCCTTT | (CTT)6 | 130–142 | 50 | MH844987 |
R:TAGACCAAGAGCCAAATAAACCA | |||||
AD24 | F (TAMRA):GCGAGATGGAAATGACAAATTCT | (GCA)6 | 108–117 | 50 | MH844988 |
R: ATCCCACCATTTCCTCATTAAGT |