Sequence type | Gene name | Primer sequences |
---|---|---|
Precursor cloning | cgo-MIR390a | F: gagaacacgggggactctagaAAAAGCAGGGAGAAATCCTTAAAG |
 | R: ataagggactgaccacccgggGAGAGCAGGAAGAACCAATAAAACT | |
cgo-MIR390b | F: gagaacacgggggactctagaACAAGTATGGGAGAACCATTAAAGC | |
 | R: ataagggactgaccacccgggAAGAGCAAGAAGAACCCATAAAACTC | |
cgo-MIR390c | F: gagaacacgggggactctagaAATGTATGGGAGAACCATTAAAGCT | |
 | R: ataagggactgaccacccgggAGAGCAGGAAGAACCAATAGAACTCA | |
Reverse transcription | cgo-miR390a-5p | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTGGCGC |
cgo-miR390a-3p.1 | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCTCAGG | |
cgo-miR390a-3p.2 | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAAAACT | |
Fluorescence quantification | cgo-miR390a-5p | F: CGCGAAGCTCAGGAGGGATA |
 | R: AGTGCAGGGTCCGAGGTATT | |
cgo-miR390a-3p.1 | F: GCGCGTGTGACGCTATCTAT | |
 | R: AGTGCAGGGTCCGAGGTATT | |
cgo-miR390a-3p.2 | F: CGCGCGGCTATCTATCCTG | |
 | R: AGTGCAGGGTCCGAGGTATT | |
18S | F: GGTCCTATTGTGTTGGCT | |
 | R: TCGCAGTGGTTCGTCT | |
U6 | F: GGTGCTAAGAAGAGGAAGAAT | |
 | R: CTCCTTCTTTCTGGTAAACGT |