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Table 2 List of primers sequences used for the experiments

From: miR390 family of Cymbidium goeringii is involved in the development of reproductive organs in transgenic Arabidopsis

Sequence type

Gene name

Primer sequences

Precursor cloning

cgo-MIR390a

F: gagaacacgggggactctagaAAAAGCAGGGAGAAATCCTTAAAG

 

R: ataagggactgaccacccgggGAGAGCAGGAAGAACCAATAAAACT

cgo-MIR390b

F: gagaacacgggggactctagaACAAGTATGGGAGAACCATTAAAGC

 

R: ataagggactgaccacccgggAAGAGCAAGAAGAACCCATAAAACTC

cgo-MIR390c

F: gagaacacgggggactctagaAATGTATGGGAGAACCATTAAAGCT

 

R: ataagggactgaccacccgggAGAGCAGGAAGAACCAATAGAACTCA

Reverse transcription

cgo-miR390a-5p

GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTGGCGC

cgo-miR390a-3p.1

GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCTCAGG

cgo-miR390a-3p.2

GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAAAACT

Fluorescence quantification

cgo-miR390a-5p

F: CGCGAAGCTCAGGAGGGATA

 

R: AGTGCAGGGTCCGAGGTATT

cgo-miR390a-3p.1

F: GCGCGTGTGACGCTATCTAT

 

R: AGTGCAGGGTCCGAGGTATT

cgo-miR390a-3p.2

F: CGCGCGGCTATCTATCCTG

 

R: AGTGCAGGGTCCGAGGTATT

18S

F: GGTCCTATTGTGTTGGCT

 

R: TCGCAGTGGTTCGTCT

U6

F: GGTGCTAAGAAGAGGAAGAAT

 

R: CTCCTTCTTTCTGGTAAACGT