Fig. 4
From: Plastid retrograde regulation of miRNA expression in response to light stress
Regulation of pri-miRNA by β-CC-dependent 1O2 signaling. a Scheme represents the β-CC-dependent 1O2 signaling pathway induced in HL conditions. β-CC is formed in PSII as a result of β-carotene oxidative breakdown under HL conditions. MBS1 is a downstream component that transduces stress information to the nucleus, where it influences the expression of singlet oxygen responsive genes (SORGs). b qRT-PCR for the DRP gene and c pri-miR163 and pri-miR840 in control plants (0.00) and after β-CC treatment (0.05 ml and 1.00 ml) for 4 h. d TT-qRT PCR for miR163 and miR840 level in plants treated by β-cc. ctrl- control plants, trt—plants treated with 1.0 ml of β-CC. Transcript levels were normalized with respect to sno85 and sno101. e qRT-PCR for pri-miR163 and pri-miR840 in Col-0 and mbs1 plants. LLc-control plants; HL-plants exposed to HL for 2 h; LLr-plants exposed to HL for 2 h and subsequent recovery in LL for 4 h. Transcript levels were normalized with respect to the PP2A and UPL7 genes. Asterisks indicate significant differences according to the Tukey HSD test at the level of * ≤ 0.05, ** ≤ 0.01, *** ≤ 0.001. Mean values ± SDs (n = 3), were provided