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Fig. 3 | BMC Plant Biology

Fig. 3

From: Plastid retrograde regulation of miRNA expression in response to light stress

Fig. 3

Regulation of pri-miRNA expression is not dependent on EX1-dependent 1O2 signaling. a The Fv/Fm parameter measured in control (ctrl) plants (grown in constant light (CL)) and treated (trt) plants (plants grown for 2 weeks in CL, placed for 12 h in darkness, and re-exposed for 2 h to LL) of the Col-0, ex1, flu, and flu/ex1 genotypes (n = 9–10). b qRT-PCR of the DRP gene in the Col-0, ex1, flu, and flu/ex1 genotypes in ctrl and trt plants. c qRT-PCR for pri-miR163 and pri-miR840 in the Col-0, ex1, flu, and flu/ex1 genotypes in ctrl and trt plants. d qRT-PCR for pri-miR163 and pri-miR840 in Col-0 and ex1 plants LLc-control plants; HL-plants exposed to HL for 2 h; LLr-plants exposed to HL for 2 h and subsequent recovery in LL for 4 h. Transcript levels were normalized with respect to the PP2A and UPL7 genes. Asterisks indicate significant differences according to Tukey’s HSD test at the level of * ≤ 0.05, ** ≤ 0.01 and *** ≤ 0.001. In (b) and (c), * indicates significance within the same genotype, while ^ indicates comparison to Col-0 within the same conditions. Mean values ± SDs (n = 3) were provided

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