Fig. 6
From: Wound-induced signals regulate root organogenesis in Arabidopsis explants
Effects of EGTA on gene expressions during root organogenesis. (A-D) Expression of genes related to root organogenesis. Leaf explants from the 9-day-old Col-0 seedlings were incubated on B5-agar plates containing 0.5 mM EGTA for the indicated time periods. Leaf explants were harvested and expression of genes related to auxin biosynthesis (A), auxin transport (B), auxin perception (C), and root organogenesis (D) was analyzed using RT-qPCR. Biological triplicates were averaged. Letters indicate groups that are statistically significantly different from each other (P < 0.05, Tukey’s test). Whiskers indicate SD. (E) Spatial expression patterns of WOX11 in the leaf explants. Leaf explants from the 9-day-old WOX11pro:GUS transgenic seedlings were incubated on B5-agar plates containing 0.5 mM EGTA for the indicated time periods. Relative intensity of GUS signals near the wound site was measured using ImageJ software. Nine biological replicates were averaged and statistically analyzed using Student’s t-test (*, P < 0.05; difference from Mock). Scale bars indicate 0.5 mm. Whiskers indicate ± SD