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Fig. 4 | BMC Plant Biology

Fig. 4

From: Wound-induced signals regulate root organogenesis in Arabidopsis explants

Fig. 4

Auxin and ROS independently induce root organogenesis in the leaf explants. Whiskers indicate ± SD. A Effects of auxin on root organogenesis in DPI-treated leaf explants. Leaf explants from 9-day-old Col-0 seedlings were incubated on B5-agar plates containing 1 µM DPI with or without 0.1 µM NAA. Biological triplicates were averaged and statistically analyzed using Student’s t-test (*, P < 0.05; difference from Mock). Scale bars indicate 1 cm. Each replicate contains 20–26 explants. B-C Auxin responses in the leaf explants after DPI treatment. Leaf explants from the 9-day-old DR5rev:GFP transgenic seedlings were incubated on B5-agar plates containing 1 µM DPI for the indicated time periods. GFP fluorescence and chlorophyll autofluorescence were analyzed using confocal microscopy (B). Scale bars indicate 0.2 mm. Relative intensity of GFP signals near the wound site of the explants was measured using ImageJ software (C). Four biological replicates were averaged and statistically analyzed using Student’s t-test (*, P < 0.05; difference from Mock)

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