Fig. 4

Validation of QTL effects and additive interactions for QTLs Pbc1-3 on selected RILs. Twelve RILs (N = 3) with contrasting genotypes at the peak markers of QTLs Pbc1–3 were selected randomly for a second phenotypic evaluation with P. brassicae egg clutches. RILs are grouped by genotype at the peak markers to show pairwise effects and additive interactions between QTLs. A) interaction between Pbc1 (BrID11121) and Pbc2 (BrID90099). B) interaction between Pbc1 and Pbc3 (BrID90095). Blue box with “L” = L58 allele, red box with “R” = R-o-18 allele. Boxplots represents the interquartile range (1st and 3rd quantile) and the median. White diamonds represent mean cell death of each QTL genotype. Letters report differences in mean size of HR-like cell death between haplotypes (Tukey’s HSD test, P < 0.01)