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Fig. 1 | BMC Plant Biology

Fig. 1

From: Genetic analysis reveals three novel QTLs underpinning a butterfly egg-induced hypersensitive response-like cell death in Brassica rapa

Fig. 1

Phenotypic variation in hypersensitive response (HR)-like cell death size between ten B. rapa homozygous lines. A Cell death induced by 10–15 P. brassicae eggs. B Differential response on DH lines R-o-18 and L58 leaves underneath P. brassicae eggs. C Cell death upon spot-inoculation with 5 μl droplets of P. brassicae egg wash. D Differential response on DH lines R-o-18 and L58 leaves at egg washed-treated spots. For each accession, N = 6–10 plants were used for both experiments, each plant was treated with eggs or egg wash on two leaves. Cell death size was quantified using a custom image-based phenotyping protocol. Each plant was assigned the cell death of the most severe spot. Boxplots represents the interquartile range (1st and 3rd quantile) and the median, each dot represents a single plant. Letters report differences in mean size of HR-like cell death between accessions (Tukey’s HSD test, P < 0.01). Broad-sense heritability (H2) is indicated at top right corner of each graph. Magnification bars inside photos = 1 mm

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