Fig. 5
From: The heat is on: a simple method to increase genome editing efficiency in plants
Base editing efficiency increases after heat shock. A Occurrence of pds3 phenotype in 16 independent lines expressing PcUBI::APOBEC1::Cas9D10A:G7T and targeting PDS3-7 grown under control (C) or heat stress (3xHS) conditions. Lines that show an increase in number and/or severity of pds3 phenotypes after 3xHS are indicated with *. n=50 per line per treatment. B Occurrence of pds3 phenotype in 17 independent lines expressing PcUBI::APOBEC::Cas9:D10A:G7T and targeting PDS3-9 grown under control (C) or heat stress (3xHS) conditions. Lines that show an increase in number and/or severity of pds3 phenotypes after 3xHS are indicated with *. n=50 per line per treatment. C Quantification of base editing. Percentage of C-to-T base editing was calculated using EditR (Kluesner et al., 2018). n=26-33 per target per treatment. ***: P<0,001; **: P<0,01 (One-Way ANOVA; Kruskal-Wallis test). D Indel frequency in base editing lines targeting PDS3-7 or PDS3-9. n=20-24 per target per treatment