Fig. 4
From: The heat is on: a simple method to increase genome editing efficiency in plants
Inheritability of 3xHS induced mutations. A Experimental set-up to investigate inheritability of 3xHS induced mutations. B For each T-DNA-free T2 progeny analysed, the growth condition of T1 plants is indicated (Control or 3xHS). For each T2 progeny analysed, the percentage of plants with a glabrous or pds3 phenotype (GL1-1, GL1-2 and PDS3-1) or displaying resistance to allyl alcohol treatment (ADH1-2) is indicated. n=25-50 per T2 plants per T1 plant. The number of T2 lines exhibiting mutant phenotypes is given for each target and condition below the graph. For Cas12a lines targeting PDS3, T3 progeny was scored in the same way as the T2 Cas9 lines. C Indel frequency in T2 lines targeting GL1-1 and exhibiting a glabrous phenotype. T1 growth conditions is indicated n=15 per T1 treatment