Fig. 3
From: The heat is on: a simple method to increase genome editing efficiency in plants
Indel efficiency increases after heat shock, irrespective of the target gene. T1 plants were used containing LbCas12a or Cas9 and targeting PDS3, GLABRA1 (GL1-2), At2g22460, IMMUTANS1 (IM1), VARIEGATED1 (VAR1-1 and VAR1-2), TRANSPORT INHIBITOR RESPONSE 1 (TIR1-1) or At4g12990. DNA was extracted for four to eight randomly selected individuals for each line and treatment, PCR products amplified from targeted loci were sequenced and analyzed using ICE (https://ice.synthego.com). The KO-score is given for each sample, indicating indels that result in a frameshift or are 21+bp in length. Lines indicate mean KO-score per sample per treatment. n=4-8 per sample per treatment