Fig. 2
From: The heat is on: a simple method to increase genome editing efficiency in plants
Heat stress increases genome editing efficiency in Nicotiana tabacum. A Phenotype of in vitro grown segregating PcUBI::SpCas9::G7T lines under control conditions or 3xHS regime. Picture taken 14 days after the last heat shock. Scale=1cm. B T2 segregating lines of PcUBI::Cas9::Pea3AT targeting PDS3 with one of two gRNAs were grown under control conditions (C) or subjected to 3xHS (HS). pds3phenotypes were scored according to the severity (albino, mosaic or spots) 14 days after final HS for each line and condition. Lines that show an increase in number and/or severity of pds3 phenotypes after 3xHS are indicated with *. n=50 for each line and condition