Fig. 7

Expression and transient transformation assays identify AmMYB10 as an activator of anthocyanin biosynthesis leaves. A Real-time RT-PCR analysis showing increased expression of AmMYB10 during fruit development. Expression of AmACTIN was used as a reference gene. Error bars are standard error of three independent biological replicates. B A representative Nicotiana benthamiana leaf image infiltrated with AmbHLH33, AmMYB10, AmMYB114 and AmMYB114s agrobacterium culture. Noting weak anthocyanin accumulation after AmMYB10 infiltration. C Combination of AmMYB10 with AmbHLH33 (MYB10 + b) enhances anthocyanin accumulation, while the combination of AmbHLH33 with either AmMYB114 or AmMYB114s does not activate anthocyanin biosynthesis. D The combination of AmMYB10 with AmMYB114 or AmMYB114s does not affect anthocyanin accumulation. Digital photographs were taken 6 days after infiltration. Bar = 2 cm