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Fig. 2 | BMC Plant Biology

Fig. 2

From: GIF1 controls ear inflorescence architecture and floral development by regulating key genes in hormone biosynthesis and meristem determinacy in maize

Fig. 2

Overexpressing GIF1-GFP rescues the defective phenotypes on the ear inflorescence of the gif1 mutant under ZZC01 background. A and B Whole plant (A) and ear inflorescence (B) of the gif1-1 mutant. The gif1-1 mutant produced highly branched inflorescence (B). C and D Whole plant (C) and ear inflorescence (D) of transgenic line OE2 overexpressing GIF1-GFP. E and F Whole plant (E) and ear inflorescence (F) of the complemented line. Gene-specific molecular markers were used to genotype the gif1gif1/OE2 individuals in the progeny families derived from + /gif1 (ZZC01) × GIF1-GFP-OE2 (ZZC01), which are homozygous genotype at the gif1 locus and are expressing the GIF1-GFP. G Plant height, ear height and leaf length of transgenic line OE2 overexpressing GIF1-GFP and corresponding non-transgenic line (NT) under ZZC01 background. H Plant height, ear height and leaf length of complementation individuals with gif1gif1/OE2, wild-type individuals with GIF1/gif1 or GIF1/GIF1 genotype, and gif1gif1 individuals identified from the progeny families derived from + /gif1(ZZC01) × GIF1-GFP-OE2 (ZZC01). The scale bars = 500 μm in (B), (D) and (F), and 10 cm in (A), (C) and (E). Sample size = 30 in (G) and (H). The column shows mean ± standard deviation (s.d.), the statistical significance was estimated using a Student’s t-test. ** P < 0.01. ns: not significant statistically

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BMC Plant Biology

ISSN: 1471-2229

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