Fig. 6
From: Ethylene-induced potassium transporter AcKUP2 gene is involved in kiwifruit postharvest ripening
AcKUP2 gene expression and promoter activity analysis. A The time course of ethephon-induced expression of AcKUP2 gene. Kiwifruit-flesh cubes (1 cm3) were prepared from the fruit pulp and immediately immersed in 200 mL of equilibration solution (ethephon-treated: 50 mM ethephon, water-treated: control). Freshly cut kiwifruit discs were washed by gently stirring for 0, 1, 3, 5, 7, and 9 h in the equilibration solution, and gene expression was analysed by qRT-PCR. The data are expressed as mean ± SD. The asterisks indicate a significant difference according to student’s t test (**P < 0.01). B Schematic representation of AcKUP2 promoter cloned in pCAMBIA1391 vector (promoterless vector) at Pst I and BamH I sites for measuring GUS activity and field-infiltration. B Inflorescence stem with GUS histochemical staining and GUS activity in tomato. Each date represents the mean of at least three replications and values are represented as mean ± SD. Bars with the same letter are not significantly different at P < 0.01